1. Check the balance is level using the level indicator. If the bubble is not in the centre adjust the level, change the pedestal, until the bubble is in the centre of the inner circle.
2. Wear gloves and a lab coat.
3. Clean the balance by dusting off the stage and surrounding area.
4. Dust masks or face shields are appropriate when there is a risk of exposure to airborne particulates.
5. Use safety glasses with side shields and goggles, or a face shield depending on the material properties, volume, and the chances of spills or splashes.
6. Respirators are for high risk exposures to gases/vapors or particulates. Use must be preceded by assessment, medical clearance, fit testing and training.
1. Weighing must be done in a chemical fume hood, or
2. If a balance/scale cannot be used in the fume hood, pre-weigh a test tube or other covered vessel and add the compound inside the hood. Re-weigh to calculate the amount added, then add solvent to attain desired concentration
liquid
1. Perform all dilutions and aliquotting in a fume hood.
2. Always use different sizes of spatulas and weighing boats to deal with all amounts of chemicals.
3. Any spill must be cleaned immediately!
4. Spill-neutralizing agent(s), if applicable, must be available and personnel must be knowledgeable as to their proper use.
5. Please sign in on the log book
1. Microbial identification (16SrRNA and ITS based)
2. Gene amplification
3. Disease diagnostics
1. Be careful with PCR machine lid. Gently close, it can be damaged.
2. Check minimum volume of reaction compatible with this machine.
3. During handling of machine wear gloves.
4. Turn PCR machine off when you are done using.
5. Make sure PCR heater block is clean before you start a run. Check each tube receptacle before you start.
6. In case number of samples are less, distribute tubes evenly across block so lid will seat flat against top of tubes for even heating and sealing.
7. Please sign in on the log book.
1. For laboratory experiments including without toxic, infectious, radioactive, or sensitizing materials.
2. Bacterial and Fungal culture only
1. The cabinet must be on at least 5 minutes before starting biohazard work.
2. Never work with the cabinet UV light source illuminated.
3. The researcher should wear a closed-front lab coat (or surgical gown) and gloves.
4. Perform planning, start-up, wipe down, loading, work practice, purging, unloading, final wipe down and shutdown conscientiously in accordance with microbiological techniques.
5. Do not overload the containment area or block front, side, or rear air grills which will reduce or restrict air flow and compromise product or personal protection.
6. Do not use electric fans in the room when the biological safety cabinet is operating.
7. Decontaminate the cabinet with an appropriate disinfectant at the end of each operation.
8. Check periodic maintenance such as check HEPA filter, checking for leaks in the cabinet, and testing airflow velocity, to ensure it is operating safely.
9. Please sign in on the log book.
1. DNA and RNA analysis
2. Post PCR product analysis
1. Keep the apparatus away from sinks or other water sources.
2. Inspect the electrophoresis chamber for buffer leaks, caused by crazing or cracks in the plastic. Loss of buffer can lead to electrical arcing and fires.
3. If using ethidium bromide, have a hand-held UV light source available in the laboratory. Check working surfaces after each use.
4. Ultraviolet (UV) light boxes and handheld lamps used in visualizing ethidium bromide gels and pose potential exposures to UV radiation.
5. Consider using ethidium bromide substitutes such as SYBR Safe DNA Gel Stain or any other alternate
6. Decontaminate surfaces with ethanol.
7. Dispose of all materials in biohazardous waste container.
8. Do not run electrophoresis equipment while unattended.
9. The user should always turn off the main power supply switch and wait 15 seconds (the voltage may not be completely discharged from internal capacitors) before making any disconnection or connections. After use, turn the power supply off before disconnecting both leads from the power supply.
10. Please sign in on the log book.
The water distiller purifies water by evaporation and condensation of the steam. It removes organic as well as inorganic material. However, it does not produce sterile water because spores may not be destroyed during the process and because the equipment does not guarantee sterile conditions of collection and storage.
1. Do not run water distillation unit while unattended.
2. Please insure there are no water leaks from the hose connection and the drain water flows freely
3. Before its operation, check the flow rate of the water supply is sufficient
4. Insure the safety cut-out should switch off the electricity and the water supply when water level is low in main reservoir.
5. Please sign in on the log book.
1. Plant cell growth
2. Insect studies
3. Fermentation studies
4. Bacterial culturing
5. Mycology studies
1. The use of any hazardous materials in this equipment must be monitored by safety officer or some other suitably qualified individual.
2. BOD Incubator not suitable for drying, evaporating and burning-in of paints, enamels or similar materials whose solvents may produce an inflammable mixture with air.
3. Avoid usage of chemical which can results the formation of inflammable gas/air mixtures either within the equipment or in the immediate surroundings of the equipment.
4. Properly clean the dust inside the equipment to avoid short circuits or damage of electronics.
5. In order to ensure good ventilation within equipment, the space for placing loads should not exceed half volume of workroom.
6. Keep sufficient spacing of the load inside the equipment to ensure proper ventilation within the equipment. Avoid any load on the floor, against the side wall or underneath the ceiling of the equipment.
7. Improper loading may increase the time on reaching the set temperature.
8. Please sign in on the log book.
1. DNA extraction
2. Plasmid DNA extraction
3. Protein extraction
4. Extraction of blood cells
1. Maintain proper balance rotors during runs, especially when rotors are only partially loaded with tubes or plates.
Make sure the tubes for experiments are specified for use with your centrifuge rotor. Use non-breakable materials such as PP, PE, and the like; avoid glass tubes.
2. Incorrect loading can reduce the lifetime of the rotor, and uncontrolled, heavy vibration can lead to permanently damage to machine/ an imbalance can lead to a rotor crash and even can injure you or someone else.
3. Balance your tubes according to their weight and opposing tubes should be the same type and filled with the same mass.
4. If the centrifuge begins to shake or wobble, stop it immediately. A little vibration is normal, but excessive amounts can mean danger.
5. For aerosol-tight centrifugation, both aerosol-tight caps or lids and suitable vessels must be used.
Make sure the vessel/tubes are closed and remain leak-tight.
6. Avoid that the liquid in the vessel does not touch the vessel lid therefore use only use two-thirds or 80% of the maximum filling volume.
7. Avoid spillage in the form of droplets. These droplets can become airborne during centrifugation and thereby form aerosols.
8. Do not exceed the prescribed speed limits of rotors, it can lead to tube breakage or even a rotor crash.
9. If a tube break or leak, do not open the centrifuge for at least 30 minutes after the run.
10. Good maintenance and regular checks of the centrifuge, rotors, and equipment is necessary for ensuring safety and preventing system failure.
The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring six decoupled channels and white LED) and featuring six independent Veriflex temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.
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